Journal: Frontiers in Public Health

Article Title: Evaluation of the radiomodifying effects of Ginkgo biloba in radioresistant triple negative breast cancer cells

doi: 10.3389/fpubh.2026.1952136

Figure Lengend Snippet: Concentration- and time dependent effects of Ginkgo biloba (GB) treatment, alone and in combination with 3 Gy irradiation, on the viability of MDA-MB-231 cells as determined by MTT assay. Cell viability was assessed at 24 h (A) , 48 h (B) and 72 h (C) following treatment with increasing concentrations of GB. Data are presented as mean ± SD from three independent biological experiments. Asterisks indicate significant differences compared with the corresponding untreated control (0 μg/mL GB): p < 0.05 (*) and p < 0.01 (**).

Article Snippet: The human triple-negative breast cancer (TNBC) parental cell line MDA-MB-231 (HTB-26, ATCC, Manassas, VA, USA) was cultured in DMEM supplemented with 10% heat-inactivated FBS and 1% penicillin–streptomycin.

Techniques: Concentration Assay, Irradiation, MTT Assay, Control

Journal: Frontiers in Public Health

Article Title: Evaluation of the radiomodifying effects of Ginkgo biloba in radioresistant triple negative breast cancer cells

doi: 10.3389/fpubh.2026.1952136

Figure Lengend Snippet: Effects of Ginkgo biloba (GB) treatment, alone and in combination with 3 Gy irradiation, on intracellular reactive oxygen species (ROS) generation in MDA-MB-231 cells, assessed by flow cytometry using CellROX ® Green staining. Cells treated with TBHP (positive control) and untreated cells (negative control) are shown in panel (A) . Cells were treated with increasing concentrations of GB (0–300 μg/mL), and the percentage of live cells (B) and intracellular ROS levels (C) were evaluated in non-irradiated and irradiated groups. Data are presented as mean ± SD from three independent biological experiments.

Article Snippet: The human triple-negative breast cancer (TNBC) parental cell line MDA-MB-231 (HTB-26, ATCC, Manassas, VA, USA) was cultured in DMEM supplemented with 10% heat-inactivated FBS and 1% penicillin–streptomycin.

Techniques: Irradiation, Flow Cytometry, Staining, Positive Control, Negative Control

Journal: Frontiers in Public Health

Article Title: Evaluation of the radiomodifying effects of Ginkgo biloba in radioresistant triple negative breast cancer cells

doi: 10.3389/fpubh.2026.1952136

Figure Lengend Snippet: Effects of Ginkgo biloba (GB) treatment on the percentage of live MDA-MB-231 cells assessed by apoptosis assay using flow cytometry following exposure to 3 Gy irradiation. Cells were treated with increasing concentrations of GB (0–300 μg/mL), and viable cell populations were analyzed at 24 h (A) , 48 h (B) , and 72 h (C) hours in both non-irradiated and irradiated groups. Data are presented as mean ± SD from three independent biological experiments.

Article Snippet: The human triple-negative breast cancer (TNBC) parental cell line MDA-MB-231 (HTB-26, ATCC, Manassas, VA, USA) was cultured in DMEM supplemented with 10% heat-inactivated FBS and 1% penicillin–streptomycin.

Techniques: Apoptosis Assay, Flow Cytometry, Irradiation

Journal: Journal of the American Chemical Society

Article Title: A Common Small-Molecule Inhibitor Targeting Multiple Siglecs Associated with Immune Checkpoint Suppression of Cytotoxicity and Phagocytosis

doi: 10.1021/jacs.6c15475

Figure Lengend Snippet: Siglec-10 binding to R3 and derivatives. (a) Structures of glycans R1 to R5. (b) Glycan array results of Siglec-10- Fc binding to glycans R1 to R5. Data are mean ± SD (c) Flow cytometry results of Siglec-10 binding to MCF-7 in the presence of R1 to R5 (200 μM), respectively. (d) Siglec-10 binding to MCF-7 at different R3 concentrations. (e) Siglec-10 binding to MCF-7 at different R5 concentrations.

Article Snippet: Human breast cancer cell lines MCF-7 (HTB-22, the American Type Culture Collection, WT) and CD24-knockout MCF-7 (KC-1753, KYinno Biotechnology, CD24-KO) were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS), 1 × nonessential amino acids (Gibco) and 1 × antibiotic-antimycotic (Gibco).

Techniques: Binding Assay, Glycoproteomics, Fc Binding, Flow Cytometry

Journal: Journal of the American Chemical Society

Article Title: A Common Small-Molecule Inhibitor Targeting Multiple Siglecs Associated with Immune Checkpoint Suppression of Cytotoxicity and Phagocytosis

doi: 10.1021/jacs.6c15475

Figure Lengend Snippet: In vitro phagocytosis assay with flow cytometry. (a) Phagocytosis of M0-like, M1-like, or M2-like (IL-10-/IL-4-induced) macrophages to MCF-7. Normalized phagocytosis of (b) M0-like, (c) M1-like, (d) M2-like (IL-10-induced), and (e) M2-like (IL-4-induced) macrophages. Phagocytoses were normalized to the highest value of each experiment ( n = 3 donors). (f) Cytotoxicity of NK cells to MCF-7 in the presence of 2 mM R3 or PBS control. Cytotoxicity activities were normalized to the highest value of each experiment ( n = 3 donors). Statistical significance was determined using a paired, two-tailed t test on the original raw data across independent biological replicates ( n = 3 donors; * p < 0.05, ** p < 0.01) Data are mean ± SEM.

Article Snippet: Human breast cancer cell lines MCF-7 (HTB-22, the American Type Culture Collection, WT) and CD24-knockout MCF-7 (KC-1753, KYinno Biotechnology, CD24-KO) were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS), 1 × nonessential amino acids (Gibco) and 1 × antibiotic-antimycotic (Gibco).

Techniques: In Vitro, Phagocytosis Assay, Flow Cytometry, Control, Two Tailed Test